Coding

Part:BBa_K4339001:Design

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-08)


Alanine tRNA synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
    Illegal AgeI site found at 709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1090
    Illegal SapI.rc site found at 1138
    Illegal SapI.rc site found at 1288


Design Notes

The sequence was modified to remover forbidden restriction enzyme sites.


Source

E. coli MG1655 K-12.

References